Process for making hepatitis b core protein modulators

ABSTRACT

The present disclosure provides, in part, a process for preparing compounds (I) having allosteric effector properties against Hepatitis B virus Cp.

RELATED APPLICATION

This application claims priority to and the benefit of U.S. provisional application No. 62/470,560, filed on Mar. 13, 2017, which is incorporated by reference in its entirety.

BACKGROUND

Hepatitis B (HBV) causes viral hepatitis that can further lead to chronic liver disease and increase the risk of liver cirrhosis and liver cancer (hepatocellular carcinoma). Worldwide, about 2 billion people have been infected with HBV, around 360 million people are chronically infected, and every year HBV infection causes more than one half million deaths. HBV can be spread by body fluids: from mother to child, by sex, and via blood products. Children born to HBV-positive mothers may also be infected, unless vaccinated at birth.

The hepatitis virus particle is composed of a lipid enveloped studded with surface protein (HBsAg) that surrounds the viral core. The core is composed of a protein shell, or capsid, built of 120 core protein (Cp) dimers, which in turn contains the relaxed circular DNA (rcDNA) viral genome as well as viral and host proteins. In an infected cell, the genome is found as a covalently closed circular DNA (cccDNA) in the host cell nucleus. The cccDNA is the template for viral RNAs and thus viral proteins. In the cytoplasm, Cp assembles around a complex of full-length viral RNA (the so-called pregenomic RNA or pgRNA and viral polymerase (P). After assembly, P reverse transcribes the pgRNA to rcDNA within the confines of the capsid to generate the DNA-filled viral core.

At present, chronic HBV is primarily treated with nucleos(t)ide analogs (e.g. entecavir) that suppress the virus while the patient remains on treatment but do not eliminate the infection, even after many years of treatment. Once a patient starts taking nucleotide analogs most must continue taking them or risk the possibility of a life threatening immune response to viral rebound. Further, nucleos(t)ide therapy may lead to the emergence of antiviral drug resistance.

The only FDA approved alternative to nucleos(t)ide analogs is treatment with interferon a or pegylated interferon a. Unfortunately, the adverse event incidence and profile of interferon a can result in poor tolerability, and many patients are unable to complete therapy. Moreover, only a small percentage of patients are considered appropriate for interferon therapy, as only a small subset of patients are likely to have a sustained clinical response to a course of interferon therapy. As a result, interferon based therapies are used in only a small percentage of all diagnosed patients who elect for treatment.

Thus, current HBV treatments can range from palliative to watchful waiting. Nucleos(t)ide analogs suppress virus production, treating the symptom, but leave the infection intact Interferon a has severe side effects and less tolerability among patients and is successful as a finite treatment strategy in only a small minority of patients. Accordingly, there is a clear on-going need for more effective processes for making compounds capable of treating viral infections, for example, hepatitis B.

SUMMARY

The present disclosure provides, for example, a process for preparing hepatitis B core protein modulators, e.g., a process for preparing compounds which may have allosteric effector properties against Hepatitis B virus Cp, a protein found as a dimer, a multimer, and as the protein shell of the HBV core. For example, provided herein is a process for preparing compounds which may be useful for treating viral infections, such as hepatitis B.

In certain embodiments, provided herein is a process for preparing a compound of Formula I:

wherein w, R¹, R², R³, R⁴, R⁵, R⁶, R⁷, and R⁸ are as defined below.

In certain other embodiments, provided herein is a process for preparing a compound of Formula VIII:

wherein w and A are as defined below.

DETAILED DESCRIPTION

The features and other details of the disclosure will now be more particularly described. Before further description of the present invention, certain terms employed in the specification, examples and appended claims are collected here. These definitions should be read in light of the remainder of the disclosure and as understood by a person of skill in the art. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by a person of ordinary skill in the art.

Definitions

As intended herein, the terms “a” and “an” include singular as well as plural references unless the context clearly dictates otherwise.

The term “alkyl” as used herein refers to a saturated straight or branched hydrocarbon. Exemplary alkyl groups include, but are not limited to, straight or branched hydrocarbons of 1-6, 1-4, 1-3 or 2-6 carbon atoms, referred to herein as Ci₋₆alkyl, C₁₋₄ alkyl, Ci₋₃alkyl, and C2₋₆alkyl, respectively. Exemplary alkyl groups include, but are not limited to, methyl, ethyl, propyl, isopropyl, 2-methyl-1-butyl, 3-methyl-2-butyl. 2-methyl-1-pentyl, 3-methyl-1-pentyl, 4-methyl-1-pentyl, 2-methyl-2-pentyl, 3-methyl-2-pentyl, 4-methyl-2-pentyl, 2,2-dimethyl-1-butyl, 3,3-dimethyl-1-butyl, 2-ethyl-1-butyl, butyl, isobutyl, t-butyl pentyl, isopentyl neopentyl, hexyl etc.

The term “alkenyl” as used herein refers to an unsaturated straight or branched hydrocarbon having at least one carbon-carbon double bond. Exemplary alkenyl groups include, but are not limited to, a straight or branched group of 2-6 or 3-4 carbon atoms, referred to herein as C2₋₆alkenyl, and C₃₋₄alkenyl, respectively. Exemplary alkenyl groups include, but are not limited to, vinyl, allyl, butenyl, pentenyl, etc.

The term “alkoxy” as used herein refers to a straight or branched alkyl group attached to oxygen (alkyl-O—). Exemplary alkoxy groups include, but are not limited to, alkoxy groups of 1-6 or 2-6 carbon atoms, referred to herein as Ci₋₆alkoxy, and C2₋₆alkoxy, respectively. Exemplary alkoxy groups include, but are not limited to methoxy, ethoxy, isopropoxy, etc.

The term “alkynyl” as used herein refers to an unsaturated straight or branched hydrocarbon having at least one carbon-carbon triple bond. Exemplary alkynyl groups include, but are not limited to, straight or branched groups of 2-6, or 3-6 carbon atoms, referred to herein as C2₋₆alkynyl, and C3₋₆alkynyl, respectively. Exemplary alkynyl groups include, but are not limited to, ethynyl, propynyl, butynyl, pentynyl, hexynyl, methylpropynyl, etc.

The terms “cycloalkyl” or a “carbocyclic group” as used herein refers to a saturated or partially unsaturated hydrocarbon group of, for example, 3-6, or 4-6 carbons, referred to herein as C3₋₆cycloalkyl or C4₋₆cycloalkyl, respectively. Exemplary cycloalkyl groups include, but are not limited to, cyclohexyl, cyclopentyl, cyclopentenyl, cyclobutyl or cyclopropyl.

The terms “halo” or “halogen” as used herein refer to F, Cl, Br, or I.

The terms “heteroaryl” or “heteroaromatic group” as used herein refers to a monocyclic aromatic 5-6 membered ring system containing one or more heteroatoms, for example one to three heteroatoms, such as nitrogen, oxygen, and sulfur. Where possible, said heteroaryl ring may be linked to the adjacent radical though carbon or nitrogen. Examples of heteroaryl rings include but are not limited to furan, thiophene, pyrrole, thiazole, oxazole, isothiazole, isoxazole, imidazole, pyrazole, triazole, pyridine or pyrimidine etc.

The terms “heterocyclyl” or “heterocyclic group” are art-recognized and refer to saturated or partially unsaturated 4-7 membered ring structures, whose ring structures include one to three heteroatoms, such as nitrogen, oxygen, and sulfur. Where possible, heterocyclyl rings may be linked to the adjacent radical through carbon or nitrogen. Examples of heterocyclyl groups include, but are not limited to, pyrrolidine, piperidine, morpholine, thiomorpholine, piperazine, oxetane, azetidine, tetrahydrofuran or dihydrofuran etc

The terms “hydroxy” and “hydroxy l” as used herein refers to the radical —OH.

“Treatment” as used herein includes the alleviation prevention, reversal, amelioration or control of a pathology, disease, disorder, process, condition or event including viral infection. In this context the term “treatment” is further to be understood as embracing the use of a drug to inhibit block, reverse, restrict or control progression of viral infection.

As used herein, the term “pharmaceutical composition” refers to compositions of matter comprising at least one pharmaceutical compound and optionally a pharmaceutically acceptable carrier.

As used herein, the term “pharmaceutical compound” or “drug” refers to a free compound, its therapeutically suitable salts, solvates such as hydrates, specific crystal forms of the compound or its salts, or therapeutically suitable prodrugs of the compound.

“Pharmaceutically” or pharmacologically acceptable” include molecular entities and compositions that do not produce an adverse, allergic or other untoward reaction when administered to an animal, or a human, as appropriate. For human administration, preparations should meet sterility, pyrogenicity, and general safety and purity standards as required by FDA Office of Biologies standards.

The term “pharmaceutically acceptable carrier” or “pharmaceutically acceptable excipient” as used herein refers to any and all solvents, dispersion media, coatings, isotonic and absorption delaying agents, and the like, that are compatible with pharmaceutical administration. The use of such media and agents for pharmaceutically active substances is well known in the art. The compositions may also contain other active compounds providing supplemental, additional, or enhanced therapeutic functions.

The compounds of the disclosed process may contain one or more chiral centers and, therefore, exist as stereoisomers. The term “stereoisomers” when used herein consist of all enantiomers or diastereomers. These compounds may be designated by the symbols “(+),” “(−),” “R” or “S,” depending on the configuration of substituents around the stereogenic carbon atom, but the skilled artisan will recognize that a structure may denote a chiral center implicitly. The present invention encompasses various stereoisomers of these compounds and mixtures thereof. Mixtures of enantiomers or diastereomers may be designated “(±)” in nomenclature, but the skilled artisan will recognize that a structure may denote a chiral center implicitly.

The compounds of the disclosed process may contain one or more double bonds and, therefore, exist as geometric isomers resulting from the arrangement of substituents around a carbon-carbon double bond. The symbol ═ denotes a bond that may be a single, double or triple bond as described herein. Substituents around a carbon-carbon double bond are designated as being in the “Z” or “£” configuration wherein the terms “Z” and “£” are used in accordance with IUPAC standards. Unless otherwise specified, structures depicting double bonds encompass both the “E” and “Z” isomers. Substituents around a carbon-carbon double bond alternatively can be referred to as “cis” or “trans,” where “cis” represents substituents on the same side of the double bond and “trans” represents substituents on opposite sides of the double bond.

The compounds of the disclosed process may contain a carbocyclic or heterocyclic ring and therefore, exist as geometric isomers resulting from the arrangement of substituents around the ring. The arrangement of substituents around a carbocyclic or heterocyclic ring are designated as being in the “Z” or “E” configuration wherein the terms “Z” and “E” are used in accordance with IUPAC standards. Unless otherwise specified, structures depicting carbocyclic or heterocyclic rings encompass both “Z” and “E” isomers. Substituents around a carbocyclic or heterocyclic rings may also be referred to as “cis” or “trans”, where the term “cis” represents substituents on the same side of the plane of the ring and the term “trans” represents substituents on opposite sides of the plane of the ring. Mixtures of compounds wherein the substituents are disposed on both the same and opposite sides of plane of the ring are designated “cis/trans.”

Individual enantiomers and diasteriomers of compounds of the disclosed process can be prepared synthetically from commercially available starting materials that contain asymmetric or stereogenic centers, or by preparation of racemic mixtures followed by resolution methods well known to those of ordinary skill in the art. These methods of resolution are exemplified by (1) attachment of a mixture of enantiomers to a chiral auxiliary, separation of the resulting mixture of diastereomers by recrystallization or chromatography and liberation of the optically pure product from the auxiliary, (2) salt formation employing an optically active resolving agent, (3) direct separation of the mixture of optical enantiomers on chiral liquid chromatographic columns or (4) kinetic resolution using stereoselective chemical or enzymatic reagents. Racemic mixtures can also be resolved into their component enantiomers by well known methods, such as chiral-phase liquid chromatography or crystallizing the compound in a chiral solvent. Stereoselective syntheses, a chemical or enzymatic reaction in which a single reactant forms an unequal mixture of stereoisomers during the creation of a new stereocenter or during the transformation of a pre-existing one, are well known in the art. Stereoselective syntheses encompass both enantio- and diastereoselective transformations, and may involve the use of chiral auxiliaries. For examples, see Carreira and Kvaerno, Classics in Stereoselective Synthesis, Wiley-VCH: Weinheim, 2009.

The compounds of the disclosed process can exist in solvated as w ell as unsolvated forms with pharmaceutically acceptable solvents such as water, ethanol, and the like, and it is intended that the invention embrace both solvated and unsolvated forms. In one embodiment, the compound is amorphous. In one embodiment, the compound is a single polymorph. In another embodiment, the compound is a mixture of polymorphs. In another embodiment, the compound is in a crystalline form.

The invention also embraces isotopically labeled compounds of the disclosed process which are identical to those recited herein, except that one or more atoms are replaced by an atom having an atomic mass or mass number different from the atomic mass or mass number usually found in nature. Examples of isotopes that can be incorporated into compounds of the invention include isotopes of hydrogen, carbon, nitrogen, oxygen, phosphorus, sulfur, fluorine and chlorine, such as ²H, ³H, ¹³C, ¹⁴C, ¹⁵N, ¹⁸O, ¹⁷O, ³¹P, ³²P, ³⁵S, ¹⁸F, and ³⁶Cl, respectively. For example, a compound of the invention may have one or more H atom replaced with deuterium.

Certain isotopically-labeled compounds of the disclosed process (e.g., those labeled with ³H and ¹⁴C) are useful in compound and/or substrate tissue distribution assays. Tritiated (i.e., ³H) and carbon-14 (i.e., ¹⁴C) isotopes are particularly preferred for their ease of preparation and detectability. Further, substitution with heavier isotopes such as deuterium (i.e., ²H) may afford certain therapeutic advantages resulting from greater metabolic stability (e.g., increased in vivo half-life or reduced dosage requirements) and hence may be preferred in some circumstances. Isotopically labeled compounds of the invention can generally be prepared by following procedures analogous to those disclosed in the examples herein by substituting an isotopically labeled reagent for a non-isotopically labeled reagent.

The term “therapeutically suitable salt,” refers to salts or zwitterions of pharmaceutical compounds which are water or oil-soluble or dispersible, suitable for treatment of disorders and effective for their intended use. The salts may be prepared, for instance, during the final isolation and purification of the compounds or separately by reacting an amino group of the compounds with a suitable acid. For example, a compound may be dissolved in a suitable solvent such as but not limited to methanol and water, and treated with at least one equivalent of an acid, for instance hydrochloric acid. The resulting salt may precipitate out and be isolated by filtration and dried under reduced pressure. Alternatively, the solvent and excess acid may be removed under reduced pressure to provide the salt. Representative salts include acetate, adipate, alginate, citrate, aspartate, benzoate, benzenesulfonate, bisulfate, butyrate, camphorate, camphorsulfonate, digluconate, glycerophosphate, hemisulfate, heptanoate, hexanoate, formate, isethionate, fumarate, lactate, maleate, methanesulfonate, naphthalenesulfonate, nicotinate, oxalate, pamoate, pectinate, persulfate, 3-phenylpropionate, picrate, oxalate, maleate, pivalate, propionate, succinate, tartrate, trichloroacetate, trifluoroacetate, glutamate, para-toluenesulfonate, undecanoate, hydrochloric, hydrobromic, sulfuric, phosphoric, and the like. The amino groups of a compound may also be quaternized with alkyl chlorides, bromides, and iodides such as methyl, ethyl, propyl, isopropyl, butyl, lauryl, myristyl, stearyl, and the like.

Basic addition salts may be prepared, for instance, during the final isolation and purification of pharmaceutical compounds by reaction of a carboxyl group with a suitable base such as the hydroxide, carbonate, or bicarbonate of a metal cation such as lithium, sodium, potassium, calcium, magnesium, or aluminum, or an organic primary, secondary, or tertiary amine. Quaternary amine salts may derived, for example, from methylamine, dimethylamine, trimethylamin, triethylamine, diethylamine, ethylamine, tributylamine, pyridine, N,N-dimethylaniline, N-methylpiperidine, N-methylmorpholine, dicyclohexylamine, procaine, dibenzylamine, N,N-dibenzylphenethylamine, 1-ephenamine, and N,N′-dibenzylethytenediamine, ethylenediamine, ethanolamine, diethanolamine, piperidine, piperazine, and the like.

The term “therapeutically suitable prodrug,” refers to those prodrugs or zwitterions which are suitable for use in contact with the tissues of subjects and are effective for their intended use. The term “prodrug” refers to compounds that are transformed in vivo to a pharmaceutical compound, for example, by hydrolysis in blood. The term “prodrug,” refers to compounds that contain, but are not limited to, substituents known as “therapeutically suitable esters.” The term “therapeutically suitable ester,” refers to alkoxycarbonyl groups appended to the parent molecule on an available carbon atom. More specifically, a “therapeutically suitable ester.” refers to alkoxycarbonyl groups appended to the parent molecule on one or more available aryl, cycloalkyl and/or heterocycle groups. Compounds containing therapeutically suitable esters are an example, but are not intended to limit the scope of compounds considered to be prodrugs. Examples of prodrug ester groups include pivaloyloxymethyl, acetoxymethyl, phthalidyl, indanyl and methoxymethyl, as well as other such groups known in the art. Other examples of prodrug ester groups are found in T. Higuchi and V. Stella, Pro-drugs as Novel Delivery Systems, Vol. 14 of the A.C.S. Symposium Series, and in Edward B. Roche, ed., Bioreversible Carriers in Drug Design, American Pharmaceutical Association and Pergamon Press, 1987, both of which are incorporated herein by reference.

The terms “pharmaceutically effective amount” and “effective amount”, as used herein, refer to an amount of a pharmaceutical formulation that will elicit the desired therapeutic effect or response when administered in accordance with the desired treatment regimen.

The term “modulating” encompasses, fore example, increasing, enhancing, inhibiting, decreasing, and suppressing. The terms “increasing” and “enhancing” mean to cause a net gain by cither direct or indirect means. As used herein, the terms “inhibiting” and “decreasing” encompass causing a net decrease by either direct or indirect means.

Without being bound by theory, compounds of the disclosed process may ultimately target multimerization of viral core proteins, which is central to HBV infection, where the core protein multimerizes into shell, or capsid, and/or compounds of the disclosed process may for example, ultimately target interaction of viral core proteins with other macromolecules, such as host or viral nucleic acid, host proteins, or other viral proteins. For example, compounds of the disclosed process may be considered in some embodiments CpAM—core protein allosteric modifiers. CpAM interaction with core protein can allosterically favor an assembly-active form of Cp dimer and lead to viral capsid assembly at an inappropriate time or place or lead to non-standard intersubunit interactions, all resulting in defective capsids. CpAMs may additionally or alternatively affect steps of “upstream” of capsid assembly by altering the concentrations or nature of Cp available as dimer as compared to capsid or other multimeric forms. Compounds of the disclosed process or CpAMs may, in some embodiments, noticeably affect functions upstream of viral assembly-such as modulation of covalently closed circular DNA (cccDNA) transcription, RNA stability and/or protein-protein interactions.

Hepatitis B Core Protein Modulators

In one embodiment, provided herein is a process for preparing a compound represented by Formula I:

wherein w is 0, 1, or 2;

R¹ is selected from the group consisting of Ci₋₆alkyl (optionally substituted with one, two or three halogens), phenyl, and 4-6 membered monocyclic heteroaryl; wherein phenyl and 4-6 membered monocyclic heteroaryl may optionally be substituted with one or more substituents each independently selected from R¹¹;

-   -   R¹¹ is selected from the group consisting of hydrogen, halogen,         cyano, Ci₋₆alkyl, Ci₋₆alkoxy, and —S(0)_(w)-Ci₋₆alkyl (wherein w         is 0, 1 or 2);     -   R², R³, R⁴, R⁵, R⁶, R⁷, and R⁸ are each independently selected         from the group consisting of hydrogen, halogen, Ci₋₆alkyl, and         Ci₋₆alkoxy; wherein Ci₋₆alkyl and Ci₋₆alkoxy may be optionally         substituted with one, two, three or more substituents each         independently selected from the group consisting of halogen,         hydroxyl, and Ci₋₆alkoxy;

comprising:

amidating a compound of Formula II:

to provide a compound of Formula III:

oxidizing the compound of Formula III to provide a compound of Formula IV:

cyclizing the compound of Formula IV to provide a thiazole compound of Formula V:

hydrolyzing the compound of Formula V to provide a compound of Formula VI:

and

coupling the compound of Formula VI with a tricyclic compound of Formula VII:

wherein w is 0, 1 or 2;

R², R³, R⁴, R⁵, R⁶, R⁷, and R⁸ are each independently selected from the group consisting of hydrogen, halogen, Ci₋₆alkyl, and Ci₋₆alkoxy; wherein Ci₋₆alkyl and Ci₋₆alkoxy may be optionally substituted with one, two, three or more substituents each independently selected from the group consisting of halogen, hydroxyl, and Ci₋₆alkoxy;

to provide the compound of Formula I.

In certain embodiments, amidating the compound of Formula II may comprise, for example, contacting the compound of Formula II with an amine base and an acylating agent. For example, the acylating agent may be, e.g., an acid anhydride or an acid chloride. For example, the acylating agent may be, e.g., trifluoroacetic anhydride. In one embodiment, the amine base may be, for example, triethylamine.

In other embodiments, oxidizing the compound of Formula III may comprise, for example, contacting the compound of Formula III with a chromium-based oxidizing reagent. For example, the chromium-based oxidizing reagent may be, e.g., Jones reagent.

In some embodiments, cyclizing the compound of Formula IV may comprise, for example, contacting the compound of Formula IV with a thionating reagent. For example, the thionating reagent may be, e.g., phosphorus pentasulfide.

In certain embodiments, hydrolyzing the compound of Formula V may comprise, for example, contacting the compound of Formula V with an aqueous amine base. For example, the aqueous amine base may be, e.g., aqueous methylamine.

In further embodiments, coupling the compound of Formula VI with the compound of Formula VII may be conducted, for example, in the presence of a coupling agent. For example, the coupling agent may be, e.g., hydroxybenzotriazole/ethyl-(N′,N′-dimethylamino)propylcarbodiimide hydrochloride.

In some embodiments, R², R³, R⁴, R⁵, R⁶, R⁷, and R⁸ may each independently selected, for example, from the group consisting of; e.g., hydrogen, fluoride, chloride, methyl, and methoxy.

In an embodiment, R¹ may be for example, trifluoromethyl. For example, the compound of Formula VI may be:

In certain embodiments, the compound of Formula I may be, for example:

Also provided herein is a process for preparing a compound represented by Formula VIII:

wherein w is 0, 1 or 2;

A is a 5-6 membered heteroaryl having 1, 2 or 3 heteroatoms each independently selected from the group consisting of S, N, and O; wherein said heteroaryl may optionally be substituted with one or more substituents selected from R^(f);

R^(f) is independently selected, for each occurrence, from the group consisting of hydrogen, halogen, hydroxyl, cyano, R^(a)R^(b)N—, R^(a)R^(b)N-carbonyl-, R^(a)R^(b)N—S0₂—, Ci₋₆alkyl, C₃₋₆cycloalkyl, C2₋₆alkenyl, C2₋₆alkynyl, Ci₋₆alkoxy, Ci₋₆alkyl-S(0)_(w)-, (wherein w is 0, 1 or 2), Ci₋₆alkylcarbonyl-N(R^(a))— and Ci₋₆alkoxycarbonyl-N(R^(a))—; wherein Ci₋₆alkyl, C₃₋₆cycloalkyl, C2₋₆alkenyl, C2₋₆alkynyl, Ci₋₆alkoxy, Ci₋₆alkyl-S(0)_(″)-, Ci₋₆alkylcarbonyl-N(R^(a))—, Ci₋₆alkoxycarbonyl-N(R^(a))— may be optionally substituted by one or more substituents selected from the group consisting of halogen, hydroxyl, and Ci₋₆alkoxy;

R^(a) and R^(b) are independently selected, for each occurrence, from the group consisting of hydrogen and Ci₋₃alkyl; wherein Ci₋₃alkyl may optionally be substituted by one or more substituents selected from fluorine, hydroxyl, Ci₋₃alkoxy; or R^(a) and R^(b), together with the nitrogen to which they are attached, may form a 4-6 membered heterocyclic ring;

comprising: coupling a compound of Formula IX:

with a compound of Formula X:

to provide a compound of Formula XI:

optionally oxidizing the compound of Formula XI to provide a compound of Formula XII:

wherein w is 1 or 2;

reducing the compound of Formula XII to provide a compound of Formula XIII:

wherein w is 1 or 2;

hydrolyzing the compound of Formula XIII to provide a compound of Formula XIV:

cyclizing the compound of Formula XIV to provide a tricyclic compound of Formula VII:

and amidating the compound of Formula VII to provide the compound of Formula VIII.

In certain embodiments, coupling the compound of Formula IX with the compound of Formula X may be conducted, for example, in the presence of a base. For example, the base may be, e.g., cesium carbonate.

In some embodiments, oxidizing the compound of Formula XI may comprise, for example, contacting the compound of Formula XI with aqueous hydrogen peroxide. In other embodiments, oxidizing the compound of Formula XI may comprise, for example, contacting the compound of Formula XI with sodium metaperiodate and catalytic ruthenium (III) chloride.

In one embodiment, reducing the compound of Formula XII may comprise, for example, contacting the compound of Formula XII with, e.g., Pd/C under hydrogen gas.

In certain embodiments, hydrolyzing the compound of Formula XIII may comprise, for example, contacting the compound of Formula XIII with an aqueous base. For example, the aqueous base may be, e.g., aqueous lithium hydroxide.

In another embodiment, cyclizing the compound of Formula XIV may comprise, for example, contacting the compound of Formula XIV with, e.g., carbodiimidazole.

In other embodiments, amidating the compound of Formula VII may comprise, for example, contacting the compound of Formula VII with a primary amine represented by A-CH2-NH2 in the presence of a coupling agent. For example, the coupling agent may be, e.g., hydroxybenzotriazole/ethyl-(N′,N′-dimethylamino)propylcarbodiimide hydrochloride.

In an embodiment, the primary amine may be represented by, for example:

wherein R^(f) is C₁₋₆alkyl optionally substituted by one or more fluorine atoms.

For example, the primary amine may be, e.g.:

In certain embodiments, the compound of Formula VII may be, for example:

In one embodiment, the compound of Formula VIII may be, for example:

Also provided heroin is a compound or pharmaceutically acceptable salt thereof, represented by:

prepared by the process disclosed herein.

EXAMPLES

The procedures disclosed herein can be conducted in a number of ways based on the teachings contained herein and synthetic procedures known in the art. In the description of the synthetic methods described below, it is to be understood that all proposed reaction conditions, including choice of solvent, reaction atmosphere, reaction temperature, duration of the experiment and workup procedures, can be chosen to be the conditions standard for that reaction, unless otherwise indicated. It is understood by one skilled in the art of organic synthesis that the functionality present on various portions of the molecule should be compatible with the reagents and reactions proposed. Substituents not compatible with the reaction conditions will be apparent to one skilled in the art and alternate methods are therefore indicated. The starting materials for the examples are either commercially available or are readily prepared by standard methods from known materials. At least some of the compounds identified as intermediates e.g., as part of a synthetic scheme disclosed herein, are contemplated as compounds of the invention.

In the procedures described below, it may be necessary to protect reactive functional groups (such as hydroxyl, amino, thio or carboxyl groups) to avoid their unwanted participation in the reactions. The incorporation of such groups, and the methods required to introduce and remove them are known to those skilled in the art (for example, see Greene, Wuts, Protective Groups in Organic Synthesis. 4th Ed. (2007)). The deprotection step may be the final step in the synthesis such that the removal of protecting groups affords compounds of the disclosed process. Starting materials used in the following schemes can be purchased or prepared by methods described in the chemical literature, or by adaptations thereof, using methods known by those skilled in the art. The order in which the steps are performed can vary depending on the groups introduced and the reagents used, but would be apparent to those skilled in the art.

Certain reactions of the disclosed process may be conducted in the presence of a base. Examples of such bases may include, but are not limited to, carbonates such as, e.g., Li₂C0₃, Na₂C0₃, K2CO3, Rb₂C0₃, Cs₂C0₃, MgC0₃, CaC0₃, SrC0₃, BaC0₃ and hydrates thereof; and hydroxides such as, e.g., LiOH, NaOH, KOH, Ca(OH)₂, NH₄OH and hydrates thereof; and amines such as methylamine, trimethylamine, trimethylamine, diisopropylethylamine, morpholine and morpholine derivatives.

Certain reactions of the disclosed process involving coupling an amino moiety with a carboxylic acid moiety to form an amide may be conducted in the presence of activators). Examples of such activators may include, but are not limited to, carbodiimides such as, e.g., N,N′-dicyclohexylcarbodiimide (DCC), N,N′-diisopropylcarbodiimide (DIC), and carbonyl diimidazole (CDI); and triazoles, such as, e.g., 1-hydroxy-benzotriazole (HOBt) and 1-hydroxy-7-aza-benzotriazole (HOAt). Other activators may include, but are not limited to, e.g., HBTU, HATU, HCTU, TBTU, and PyBOP.

Example 1: Synthesis of (2-(trifluoromethyl)thiazol-5-yl)methanamine

Step 1: Preparation of 1,3-bis trifluoroacetamido-2-propanol (1-2)

The title compound was prepared according to the procedure described in the Supporting Information section of Org. Lett. 2008, 10, 2935-2938 as follows. A solution of trifluoroacetic anhydride (7 kg, 4.66 mol) in 15 L of anhydrous acetonitrile was added dropwise to a solution of 1,3-diaminopropan-2-ol (1 kg, 1.11 mol) and triethylamine (3.38 kg; 3.33 mol) in anhydrous acetonitrile (13.3 L) at 0° C. After 2 hours the reaction mixture was warmed to room temperature and stirred for 14 h. The volatiles were removed, the residue dissolved in ethyl acetate (20 L), and washed sequentially with saturated aqueous sodium bicarbonate, saturated ammonium chloride, and brine. The organic layer was dried over anhydrous sodium sulfate, filtered, and concentrated to give a solid. The solids were suspended in petroleum ether (2 L), filtered, and dried to yield 2 kg of the title compound. ¹H-NMR (DMSO-J6, 300 MHZ): δ 9.43 (d, /=4.8 Hz, 2H), 7.77 (bs, 1H), 3.76 (m, 4H); LCMS-CPI m/z: 28.3.1 [M+H⁺].

Step 2: Preparation of 1,3-bis trifluoroacetamido propan-2-one (1-3)

Jones reagent (freshly prepared by dropwise addition of sulfuric acid (1.247 L) to a pre-cooled solution of chromium trioxide (1.347 kg, 13.47 mol) in water (2.695 L)) was added to a stirred solution of bis trifluoroacetamido propan-2-ol (2 kg, 7.09 mol) in acetone (20 L) at 10° C. over 3 h while maintaining the temperature at 10-35° C. The reaction mixture was stirred for an additional 3 h. warmed to room temperature and stirred overnight. The reaction mixture was filtered and the chromium salts washed with acetone (10 L). The combined filtrate was concentrated, washed with petroleum ether and dried to yield 1.49 kg of the title compound which was used without purification in the next step. ¹H-NMR (DMSO-J6, 300 MHZ): δ 9.74 (t, /=5.4 Hz, 2H), 4.25 (md, /=5.4 Hz, 4H); LCMS-CPI m/z: 281.1 [M+H⁺].

Step 3: Preparation of 2,2,2-trifluoro-N-((2-(trifluoromethyl)thiazol-5-yl)methyl) ethanethioamide (1-4)

A 20 L round bottom flask was charged with a solution of 1,3-bis trifluoroacetamido propan-2-one (800 g; 2.86 mol) in toluene and equipped with a Dean-Stark apparatus. The solution was heated at reflux for 10 h and cooled to room temperature. Phosphorous pentasulfide (19 kg; 8.57 mol) was added, and the reaction mixture was heated at reflux for 12 h. The reaction mixture was cooled to room temperature and filtered. The filter cake was washed with toluene (4 L), and the combined filtrate was concentrated to furnish 800 g of the title compound which was used without purification in the next step. ¹H-NMR (CDCl₃, 300 MHZ): δ 8.32 (bs, 1H), 7.92 (s, 1H), 5.14 (d, /=6.0 Hz, 2H): LCMS-CPI nt/z: 295.1 [M+H⁺].

Step 4: Preparation of (2-(trifluoromethyl)thiazol-5-yl)methanamine (1-5)

Aqueous methylamine (40%, 1.58 L, 20.4 mol) was added to 2,2,2-trifluoro-N-((2-(trifluoromethyl)thiazol-5-yl)methyl) ethanethioamide (600 g, 2.04 mol) in a 2 L autoclave and heated for 14 h at 80° C. The autoclave was cooled to room temperature and allowed to stand open for 2 h. The reaction mixture was extracted with dichloromethane (4×300 ml) and the combined organic layers were dried over anhydrous sodium sulfate, filtered and concentrated to give an oily residue. The residue was distilled under high vacuum at 80° C. to provide 160 g of the title compound. ¹H NMR (DMSO-de, 400 MHz): δ 7.92 (s, 1H), 6.80 (br s, 2H), 4.01 (s, 2H).

Example 2: Synthesis of (2-ethylthiazol-5-yl) methanamine Hydrochloride (2-7) by an Alternative Route

Step 1: Synthesis of ethyl 2-chloro-3-oxopropanoate (2-2)

To a stirring solution of ethyl 2-chloroacetate 2-1 (5 g, 40.98 mmol) and ethyl formate (3.03 g, 40.98 mmol) in diisopropyl ether (100 mL) under argon atmosphere was added potassium tert-butoxide (5.49 g, 45.08 mmol) portion wise for 10 min at 0° C., warmed to RT and stirred for 24 h. The reaction was monitored by TLC; after completion of the reaction, the pH of the reaction mixture was adjusted to ˜6 using 5 N HCl. The obtained solid was filtered, washed with diethyl ether (200 mL) and dried in vacuo to afford compound 2-2 (6 g) as pale brown syrup. TLC: 30% EtOAc/hexanes (R 0.2); LC-MS: 21.49%+75.58%; 149.0 (M⁺−1); (column: X-Select C-18, (50×3.0 mm, 3.5μιη); RT 0.56 min, 0.77 min. 5 Mm Aq.NH{circumflex over ( )}OAc: ACN 0.8 mL/min).

Step 2: Synthesis of ethyl 2-ethylthiazole-5-carboxylate (2-3)

To a stirring solution of compound 2-2 (lg) in ethanol (25 mL) under argon atmosphere were added propanethioamide (594 mg, 6.67 mmol), dry magnesium sulfate (4 g) at RT and heated to reflux for 24 h. The reaction was monitored by TLC; after completion of the reaction, the volatiles were removed in vacuo, diluted with EtOAc (2×100 mL). The combined organic extracts were washed with saturated sodium bicarbonate solution (2×100 mL), brine (50 mL), dried over sodium sulfate, filtered and concentrated in vacuo to obtain the crude. The crude was purified through flash column chromatography using 6% EtOAc/hexanes to afford compound 2-3 (330 mg, 27%) as brown syrup. TLC: 10% EtOAc/hexanes (Rf 0.4); ¹H-NMR (DMSO-d₆, 400 MHz): δ 8.29 (s, 1H), 4.30 (q, /=7.1 Hz, 2H), 3.04 (q, /=7.5 Hz, 2H), 1.31 (t, /=7.3 Hz, 3H), 1.29 (t, /=7.3 Hz, 3H).

Step 3: Synthesis of (2-ethylthiazol-5-yl) methanol (2-4)

To a stirring suspension of lithium aluminium hydride (205 mg, 5.40 mmol) in dry THF (15 mL) under inert atmosphere was added compound 2-3 (500 mg, 2.70 mmol) at 0° C.; warmed to RT and stirred for 16 h. The reaction was monitored by TLC; after completion of the reaction, the reaction mixture was cooled to 0° C., quenched with 20% aqueous sodium hydroxide solution (3 mL), filtered through celite and washed with EtOAc (3×100 mL). The filtrate was dried over sodium sulfate, Altered and concentrated in vacuo to afford compound 2-4 (310 mg, 80%) as pale yellow solid. TLC: 50% EtOAc/hexanes (Rf. 0.4). ¹H-NMR (CDCl₃, 400 MHz): δ 7.51 (s, 1H), 4.82 (s, 2H), 3.01 (q, /=7.5 Hz, 2H), 1.38 (t, /=7.6 Hz, 3H).

Step 4: Synthesis of 5-(chloromethyl)-2-ethylthiazole (2-5)

To a stirring solution of compound 2-4 (300 mg, 2.09 mmol) in CH2CI2 (15 mL) under inert atmosphere were added diethyl amine (0.6 mL, 4.20 mmol), DMAP (25.6 mg. 0.21 mmol) and methanesulfonyl chloride (0.19 mL, 2.51 mmol) at 0° C. warmed to RT and stirred for 2 h. The reaction was monitored by TLC; alter completion of the reaction, the reaction mixture was diluted with water (50 mL) and extracted with CH2CI2 (3×100 mL). The combined organic extracts were dried over sodium sulfate, filtered and concentrated in vacuo to afford compound 2-5 (500 mg, crude) as pale yellow syrup. TLC: 30% EtOAc/hexanes (Rf 0.8); LC-MS: 30.71%; 162.0 (M⁺+1); (column; Ascentis Express C18·(50×3.0 mm, 2.7 μm); RT 2.14 min. 0.025% Aq. TFA+5% ACN: ACN+5% 0.025% Aq. TFA, 1.2 mL/min).

Step 5: Synthesis of 5-(azidomethyl)-2-ethylthiazole (2-6)

To a stirring solution of compound 2-5 (500 mg, 2.26 mmol) in DMF (20 mL) under inert atmosphere was added sodium azide (294 mg, 4.52 mmol) at RT and heated to 80° C. for 16 h. The reaction was monitored by TLC; after completion of the reaction, the reaction mixture was diluted with ice cold water (50 mL) and extracted with EtOAc (3×100 mL). The combined organic extracts were dried over sodium sulfate, filtered and concentrated in vacuo to obtain the crude. The crude was purified through flash column chromatography using 15% EtOAc/hexanes to afford compound 2-6 (250 mg, 71%) as pale yellow syrup. TLC: 20% EtOAc/hexanes (Rfi 0.4): ¹H-NMR (CDCl₃, 400 MHz): δ 7.56 (s, 1H), 4.49 (s, 2H), 3.03 (q, /=7.6 Hz, 2H), 1.40 (t, /=7.6 Hz, 3H).

Step 6: Synthesis of (2-ethylthiazol-5-yl) methanamine hydrochloride (2-7)

To a stirring solution of compound 2-6 (250 mg, 1.48 mmol) in THF: % 0 (5:1, 12 mL) was added triphenyl phosphine (780 mg, 2.97 mmol) at RT and stirred for 2 h. The reaction was monitored by TLC: after completion of the reaction, the volatiles were removed in vacuo to obtain the crude. The obtained solid was further dried using toluene (2×5 mL) to obtain the crude amine. The crude product was dissolved in CH2CI2 (5 mL) added 4 N HCl in 1,4-dioxane (4 mL) under inert atmosphere at 0° C. and stirred for 30 min. The volatiles were removed in vacuo to obtain the crude, which was triturated with EtOAc (2 mL), diethyl ether (2 mL) and pentane (5 mL) to afford compound 2-7 (180 mg, 68%) as an off-white solid. TLC: 5% MeOH/CH₂Cl₂, (Rfi 0.2); ¹H NMR (DMSO-d₆, 500 MHz): δ 8.48 (br s, 3H), 7.74 (s, 1H), 4.25 (q, /=5.5 Hz, 2H), 2.98 (q, /=7.5 Hz, 2H), 1.28 (t, /=7.5 Hz, 3H).

Example 3: Synthesis of ll-oxo-10, 11-dihydrodibenzo [b,f] [1, 4] thiazepine-8-carboxylic acid 5,5-dioxide (3-7)

Step 1: Preparation of methyl 4-((2-(methoxycarbonyl) phenyl) thio)-3-nitrobenzoate (3-3)

To a stirred solution of methyl 4-fluoro-3-nitrobenzoate (300 g, 1.0 eq.) and methylthiosalicylate (278.7 g, 1.1 eq.) in DMF (1.8 L) was added Cs₂C0₃, (589 g, 1.2 eq.) in ₅ batches at 0 to 5° C. The reaction mixture was stirred at 0 to 5° C. for 30 min. warmed to room temperature over 2 h. and stirred at room temperature for 2 h. The reaction mixture was cooled to 10-15° C. diluted with water (26 V) and stirred for 30 min. The solids were collected by filtration, washed with water (20 V) and w-heptane (10 V), and dried under reduced pressure below 50° C. The dry solids were suspended in w-heptane (10 V) at 90-95° C. to form a slurry and cooled to 35-40° C. The solids were collected by filtration, washed with w-heptane (5 V), and dried under reduced pressure for 8 h to yield the title compound 3-3. Yield: (92.% %), Purity: 99.01%. TLC: 20% EtOAc/hexanes (R/. 0.4); ¹H NMR (CDCl₃, 400 MHz): δ 8.85 (s, 1H), 7.99-7.92 (m, 2H), 7.66-7.56 (m, 3H), 6.93 (d, /=8.6 Hz, 1H), 3.94 (s, 3H), 3.79 (s, 3H). LCMS-ESI m/z: 348 [M+H⁺].

Step 2: Preparation of methyl 4-((2-(methoxycarbonyl)phenyl)sulfonyl)-3-nitrobenzoate (3-4) Oxidation Method A:

To a stirred solution of methyl 4-((2-(methoxycarbonyl) phenyl) thio)-3-nitrobenzoate (2.3 Kg, 1.0 eq.) in AcOH (23 L, 10 V) at 85° C. was added 30% aqueous H2O2 (25 L, 10 V). The reaction mixture was stirred at 85° C. for 10 h, and a second portion of 30 aqueous % H₂0₂ (23 L, 10 V) was added and stirred at 90° C. for 10 h. A third portion of 30% aqueous H₂0₂ (11.5 L, 5 V) was added and stirred for 4 h. The reaction mixture was cooled to −15° C. and the solids were collected by filtration, washed with water (23 L, 10V) and dried under reduced pressure below 50° C. for 10 h to provide 2.14 kg of the title compound. HPLC analysis indicated a purity of 98.55%, with 0.46% of the mono-oxidized sulfoxide ¾-NMR (DMSO-J6, 500 MHZ): δ 8.51 (s, 1H), 8.39-8.41 (dd, /=1.5, 8.5 HZ, 1H), 8.16-8.18 (dd. /=2.0, 7.5 HZ, 1H), 8.13 (d, /=8.5 HZ, 1H), 7.91-7.94 (m, 2H), 7.86-7.88 (m, 1H), 3.93 (s, 3H), 3.67 (s, 3H); LCMS-ESI m/z: 397 [M+NH₄ ⁺], m/z: 402 [M+Na⁺],

Oxidation Method B:

To a stirred solution of methyl 4-((2-(methoxycarbonyl) phenyl) thio)-3-nitrobenzoate (300.0 g, 1.0 eq.) in CH₂Cl₂:acetone:H₂0 (1.5 L:1.5 L:3.0 L) at room temperature was added sodium metaperiodate (461.0 g, 2.5 eq.) and ruthenium (III) chloride (60 mg, 0.0003 eq.). After 5 h the acetone and CH2CI2 were removed by vacuum distillation below 50° C. The reaction mixture was diluted with water (1.5 L, 5 V) and stirred at room temperature for 2-3 h. The reaction mixture was filtered and the filter cake was washed with water (3.0 L, 10 V) and dried under vacuum at 50-55° C. for 12-16 h to yield 315 g of the title compound. HPLC analysis indicated a purity of 99.9%. The structure of the product was confirmed by ¹H NMR and MS to be identical to that obtained by Method A.

Step 3: Preparation of methyl 3-amino-4-((2-(methoxycarbonyl)phenyl)sulfonyl)benzoate (3-5)

To a stirred solution of methyl 4-((2-(methoxycarbonyl)phenyl)sulfonyl)-3-nitrobenzoate (100.0 g, 1.0 eq.) in EtOAc (2.5 L, 25 V) at room temperature was added 10% Pd/C (50% wet, 26.0 g, 26 wt %). The reaction mixture was stirred under ¾ in an autoclave at 10 kg/cm² for 22 h. The reaction mixture was filtered through celite, and the filter cake washed with 20% MeOH/EtOAc (100 V). The combined filtrate was concentrated to ˜2 V, diluted with w-heptane (5 V), and concentrated. A second portion of w-heptane (5 V) was added and the reaction mixture was stirred at room temperature for 2-3 h. The solids were collected by filtration, and dried under reduced pressure below 50° C. for 10 h to yield 80.0 g (87%) of the title compound 3-5. HPLC analysis indicated a purity of 97.75%. ¹H-NMR (DMSO-J6, 500 MHZ): δ 8.14 (d, /=7.5 HZ, 1H), 7.80 (t, /=7.5 HZ, 1H), 7.72 (d, /=8.0 Hz, 1H), 7.74-7.76 (m, 1H), 7.66 (d, /=7.5 Hz, 1H), 7.46 (d, /=15 Hz, 1H), 7.19-7.21 (dd, /=1.5, 8.0 Hz, 1H), 6.38 (s, 2H), 3.83 (s, 3H), 3.83 (s, 3H); LCMS-ESI m/z: 350 [M+H⁺], m/z: 372 [M+Na⁺].

Step 4: Preparation of 3-amino-4-((2-carboxyphenyl)sulfonyl)benzoic Acid (3-6)

To a stirred solution of methyl 3-amino-4-((2-(methoxycarbonyl)phenyl)sulfonyl) benzoate (1.5 Kg, 1.0 eq.) in THF (20 V) at room temperature was added a solution of LiOH.H₂0 (10 eq.) in water (10 V). After stirring at 45° C. for 12 h. the THF was removed by distillation. The reaction mixture was diluted with water (5 V) and extracted with EtOAc (2×6 V). The aqueous layer was cooled to 10° C., acidified with 5 N HCl (8 V) to pH 2, and stirred at 10-15° C. for 2 h. The aqueous layer was filtered, the filter cake washed with water (2×20 V) and heptane (2×5 V), and dried under reduced pressure below 50° C. for 26 h to afford 1.30 kg of a mixture of the title compound (65.02%) and 11-oxo-10,11-dihydrodibenzo[b,f][1,4] thiazepine-8-carboxylic acid 5,5-dioxide (34.70%), which was used without purification in the next step. Characterization of 3-amino-4-((2-carboxyphenyl)sulfonyl)benzoic acid: ¹H-NMR (DMSO-J6, 500 MHZ): δ 13.6 (bs 2H), 8.07 (d, /=7.5 HZ, 1H), 7.74-7.76 (m, 2H), 7.68 (t, /=7.5 HZ, 1H), 7.62 (d, /=7.5 Hz, 1H), 7.40 (d, /=15 Hz, 1H), 7.13-7.15 (dd, /=1.5, 8.0 Hz, 1H), 6.39 (bs, 2H); LCMS-ESI nt/z: 322 [M+H⁺], nt/z: 344 [M+Na⁺].

Step 5: Preparation of ll-oxo-10,ll-dihydrodibenzo[b,f][1,4] thiazepine-8-carboxylic acid 5,5-dioxide (3-7)

To a stirred solution of the crude product mixture of Step 5 (60.0 g, 1.0 eq.) in THF (15 V) at 15-20° C. was added CDI (3.0 eq.). The reaction mixture was warmed to room temperature and stirred for 18 h. The reaction mixture was diluted with water (15 V) and the THF was removed under reduced pressure. The reaction mixture was further diluted with water (2 V) and EtOAc (5 V) and stirred for 15-20 min. The crude suspension was filtered and the filter cake retained. The organic and aqueous layers of the filtrate were separated, and the aqueous layer was acidified with 5 N HCl (6 V) to pH 2 at 15-20° C. to obtain a precipitate. The aqueous layer was filtered, the filter cake washed with water (5 V) and dried under reduced pressure for 2 h. The two filter cakes were combined and stirred in 10% aqueous NaOH (6 V) at 0° C. for 1-2 h to obtain a clear solution. The solution was acidified to pH 2 with 5 N HCl (5-6 V) at 5-10° C. to obtain a precipitate. The precipitate was filtered, the filter cake washed with water (10 V) and dried under reduced pressure at 50-55° C. for 24 h to obtain the title compound. TLC: 5% MeOH/CH₂Cl₂ (Rf. 0.1): ¹H NMR (DMSO-d₆, 400 MHz): δ 13.65 (br s, 1H), 11.55 (s, 1H), 8.07 (d, /=8.3 Hz, 1H), 8.03-7.82 (m, 6H).

3-Step Telescoping Method Combining Steps 3, 4 and 5:

To a stirred solution of methyl 4-((2-(methoxycarbonyl)phenyl)sulfonyl)-3-nitrobenzoate (100.0 g, 1.0 eq.) in THF (0.5 L, 5 V) was added 10% Pd—C(50% wet 10.0 g, 10 wt %) at room temperature. The reaction mixture was stirred under ¾ in an autoclave at 10 kg/cm² pressure for 20 h. HPLC analysis indicated completion of the reaction and a product purity of 98.62%. The reaction mixture was filtered through a celite, tire filter cake washed with hot THF (10 V), and the combined filtrate was used directly in the next step.

To the above combined filtrate (−1.7 L) was added water (0.8 L, 10 V) and LiOH.H₂0 (96.1 g, 10 eq). After stirring at 45-50° C. for 16 to 18 h, the THF layer was separated and the aqueous layer was washed with EtOAc (5 V). The aqueous layer was acidified to pH 2 at room temperature with 5 M HCl (5-6 V), with an observed exotherm of 10-15° C. The aqueous layer was stirred at room temperature for 1-2 h and filtered. The filter cake was washed with water (15 V) and w-heptane (10 V) and dried under vacuum below 50-55° C. for 16-1.8 h to obtain a erode solid which was used directly in the next step.

To a stirred solution of the above crude material (65.0 g, 10 eq.) in THF (15 V) was added CDI (3.0 eq.) at 15-20° C. The reaction mixture was warmed to room temperature over 30 min and stirred for 18 h. The reaction mixture was diluted with water (15 V) and the THF removed under reduced pressure. The reaction mixture was diluted with water (2 V) and EtOAc (5 V), stirred for 15-20 min. filtered, and the filter cake retained. The organic and aqueous layers of the filtrate were separated, and the aqueous layer was acidified with 5 N HCl (6 V) to pH 2 at 15-20° C. to obtain a precipitate. The aqueous layer was filtered, the filter cake washed with water (5 V) and dried under reduced pressure for 2 h.

The two filter cakes were combined and stirred in 10% aqueous NaOH (6 V) at 0° C. for 1-2 h to obtain a clear solution. The solution was acidified to pH 2 with 5 N HCl (5-6 V) at 5-10° C. to obtain a precipitate. The precipitate was filtered, the filter cake washed with water (10 V) and dried under reduced pressure at 50-55° C. for 24 h to obtain 11-oxo-10, 11-dihydrodibenzo[b,f] [1,4] thiazepine-8-caboxylic acid 5,5-dioxide. ¹H-NMR (DMSO-J6, 500 MHZ): δ 13.6 (bs 2H), 11.54 (s, 1H), 8.07 (d, /=8.5 HZ, 1H), 7.97-8.01 (m, 2H), 7.94 (s, 1H), 7.84-7.93 (m, 3H), 6.39 (bs, 2H); LCMS-ESI m/z: 302 [M−H⁻].

Example 4: Synthesis of ll-oxo-N-((2-(trifluoromethyl)thiazol-5-yl)methyl)-10,ll-dihydrodibenzo[b,f][1,4]thiazepine-8-carboxamide 5,5-dioxide

To a stirred solution of 11-oxo-10, 11-dihydrodibenzo[b,f] [1,4] thiazepine-8-carboxylic acid 5,5-dioxide 3-7 (30.0 g, 1.0 eq.) in DMF (300 mL, 10 V) at 0-5° C. was added N-methyl morpholine (43.5 mL, 4.0 eq.). 2-trifluoromethyl-5-aminomethylthiazole (25.2 g, 1.4 eq.), EDC.HCI (28.4 g, 1.5 eq.) and HOBt (20.2 g, 1.5 eq.) under nitrogen. The reaction mixture was warmed to room temperature and stirred for 16 h. The reaction mixture was diluted with water (30 V) to obtain a suspension which was stirred at room temperature for 2-3 h. The suspension was filtered, the filter cake washed with water (2×10 V), and dried under vacuum for 24 h below 45° C. to obtain a crude solid. To the crude solid was added EtOAc:THF (100:10) V) and activated charcoal (15 wt %). The reaction mixture was stirred at 60-65° C. for 3 h, cooled to 35-40° C. and filtered through celite. The obtained filtrate was concentrated to 3 V, diluted with w-heptane (5 V) stirred at room temperature for 1-2 h, and filtered. The filter cake was washed with w-heptane (5 V) and dried under vacuum below 50° C. for 8-10 h to give the title compound in 75% yield. HPLS analysis indicated a purity of 99.70%. MS (m/z) calcd. for C19H12F3N3O4S2 ([M+H]⁺): 467.0; found: 468.3; ¹H-NMR (DMSO-d₆, 400 MHz): δ 11.50 (s, 1H), 9.56 (t, /=5.6 Hz, 1H), 8.08-8.04 (m, 2H), 7.98 (td, /=7.7, 0.9 Hz, 2H), 7.94-7.83 (m, 3H), 7.81 (dd. /=8.2, 1.4 Hz, 1H), 4.75 (d, /=5.5 Hz, 2H).

Example 5: l-fluoro-ll-oxo-10,ll-dihydrodibenzo[b,f][1,4]thiazepine-8-carboxylic Acid 5,5,-dioxide (5-7)

Step 1: Synthesis of methyl 2-fluoro-6-((4-(methoxycarbonyl)-2-nitrophenyl) thio) benzoate

To a stirred solution of methyl 4-fluoro-3-nitrobenzoate (4.5 g, 22.61 mmol) in DMF (100 mL) under inert atmosphere were added methyl 2-fluoro-6-mercaptobenzoate (4.6 g, crude), cesium carbonate (11 g, 33.91 mmol) at RT; heated to 60-65° C. and stirred for 2 h. The reaction was monitored by TLC, after completion of the reaction, the reaction mixture was diluted with water (600 mL) and stirred for 1 h. The precipitated solid was filtered, triturated with 10% EtOAc/hexanes (2×20 mL) and dried in vacuo to afford compound 25 (7 g, 85%) as yellow solid. TLC: 20% EtOAc/hexanes (It. 0.3): ¹H NMR (DMSO-d₆, 400 MHz): δ 8.65 (s, 1H), 8.08 (dd, /=8.6, 1.9 Hz, 1H), 7.79-7.72 (m, 1H), 7.67-7.61 (m, 2H), 7.01 (d, /=8.6 Hz, 1H), 3.88 (s, 3H), 3.72 (s, 3H).

Step 2: Synthesis of methyl 2-((2-amino-4-(methoxycarbonyl) phenyl) thio)-6-fluorobenzoate

To a stirred solution of methyl 2-((2-amino-4-(methoxycarbonyl) phenyl) thio)-6-fluorobenzoate (7.09 g, 19.17 mmol) in MeOH (200 mL) under inert atmosphere was added 10% Pd/C (3.5 g) at RT and stirred under hydrogen at 80 psi for 16 h in an autoclave. The reaction was monitored by TLC: after completion of the reaction, the reaction mixture was filtered through celite and washed with 40% MeOH/CH₂Cl₂ (3×500 mL). The combined organic extracts were dried over sodium sulfate, filtered and concentrated in vacuo to obtain the crude product which was triturated with 20% EtOAc/hexanes (200 mL) and dried in vacuo to afford methyl 2-<(2-amino-4-(methoxycarbonyl) phenyl)thio)-6-fluorobenzoate (5 g, 78%) as an off-white solid. TLC: 20% EtOAc/hexanes (R/. 0.4); ¹H NMR (DMSO-d₆, 400 MHz): δ 7.45-7.36 (m, 3H), 7.19-7.11 (m, 2H), 6.68 (d, /=7.7 Hz, 1H), 5.71 (s, 2H), 3.90 (s, 3H), 3.83 (s, 3H).

Step 3: Synthesis of 2-((2-amino-4-carboxyphenyl) thio)-6-fluorobenzoic Acid

To a stirred solution of methyl 2-((2-amino-4-(methoxycarbonyl) phenyl)thio)-6-fluorobenzoate (5 g, 14.92 mmol) in THF: H₂0 (5: 1.90 mL) was added lithium hydroxide monohydrate (3.13 g, 74.62 mmol) at RT and stirred for 16 h and heated to 80° C. for 5 h. The reaction was monitored by TLC; after completion of the reaction, the volatiles were removed in vacuo. The residue was diluted with water (200 mL) and acidified with 2 N HCl to pH˜4. The precipitated solid was filtered and dried in vacuo to afford 2-((2-amino-4-carboxyphenyl) thio)-6-fluorobenzoic acid (4 g, 87%) as an off-white solid. TLC: 30% EtOAc/hexanes (R/. 0.1), ¹H NMR (DMSO-d₆, 400 MHz): δ 12.89 (br s, 1H), 7.42-7.36 (m, 2H), 7.35-7.31 (m, 1H), 7.14-7.08 (m, 2H), 6.63 (d, /=8.0 Hz, 1H), 5.75 (br s, 2H).

Step 4: Synthesis of l-fluoro-ll-oxo-10, 11-dihydrodibenzo [b,f] [1, 4] thiazepine-8-carboxylic Acid (5-6)

To a stirred solution of 2-((2-amino-4-carboxyphenyl) thio)-6-fluorobenzoic acid (4 g, 13.02 mmol) in THF (100 mL) under inert atmosphere was added CDI (10.56 g, 65.1 mmol) at RT and stirred for 26 h. The reaction was monitored by TLC; after completion of the reaction, the volatiles were removed in vacuo. The residue was diluted with ice cold water (80 mL) and acidified with 2 N HCl to pH˜4. The precipitated solid was filtered and dried in vacuo to afford 1-fluoro-11-oxo-10, 11-dihydrodibenzo [b,f] [1,4] thiazepine-8-caboxylic acid (3.3 g, 88%) as an off-white solid. TLC: 15% MeOH/CH₂Cl₂ (Rfi 0.2); ¹H NMR (DMSO-d₆, 400 MHz): δ 13.33 (br s, 2H), 11.00 (s, 1H), 7.77 (s, 1H), 7.69-7.67 (m, 2H), 7.53-7.47 (m, 1H), 7.42-7.39 (m, 1H), 7.35-7.29 (m, 1H).

Step 6: l-fluoro-ll-oxo-10,ll-dihydrodibenzo[b,f][1,4]thiazepine-8-carboxylic Acid 5,5-dioxide (5-7)

Treating a sample of 1-fluoro-1 1-oxo-10, 11-dihydrodibenzo [b,f] [1,4] thiazepine-8-caboxylic acid under similar oxidative conditions (Method A and Method B) detailed in Example 3, the title compound was obtained.

Example 6: ll-oxo-N-((2-(trifluoromethyl)thiazol-5-yl)methyl)-10,ll-dihydrodibenzo[b,f][1,4]thiazepine-8-carboxamide 5,5-dioxide via ll-oxo-N-((2-(trifluoromethyl)thiazol-5-yl)methyl)-10,ll-dihydrodibenzo[b,f] [1,4]thiazepin-8-carboxamide (6-2)

Step 1: Synthesis of ll-oxo-N-((2-(trifluoromethyl)thiazol-5-yl)methyl)-10,ll-dihydrodibenzo[b,f][1,4]thiazepine-8-carboxamide (6-2)

The tricyclic thioether carboxylic acid 6-1 was obtained following procedures descried in Example 3 and Example 6.

Following the experimental procedure descried in Example 4 and substituting tricyclic core acid 3-7 with 6-1, the amide 6-2 was obtained. IH-NMR (DMSO-d6, 400 MHz): δ 10.77 (s, 1H), 9.38 (t, J=5.7 Hz, 1H), 8.05 (s, 1H), 7.72-7.66 (m, 3H), 7.60 (dd, J=8.0, 1.8 Hz, 1H), 7.53 ltd. J=7.1, 1.6 Hz, 1H), 7.50-7.43 (m, 2H), 4.72 (d, J=5.6 Hz, 2H); LCMS: m/z: 435.9 [M+H⁺].

Step 2: ll-oxo-N-((2-(trifluoromethyl)thiazol-5-yl)methyl)-10,ll-dihydrodibenzo[b,f][1,4]thiazepine-8-carboxamide 5,5-dioxide

The conversion of sulfide functional group in compound 6-2 to the corresponding sulfone compound was achieved by using oxidative conditions as detailed in Method A or Method B of Example 3.

Example 7: Synthesis of 9-chloro-ll-oxo-10, 11-dihydrodibenzo [b,f] [1,4] thiazepine-8-carboxylic Acid 5,5-dioxide (7-9)

Step 1: Synthesis of 1-bromo-2, 4-dichloro-3-nitrobenzene (7-2)

To a stirring solution of 1,3-dichloro-2-nitrobenzene 7-1 (5 g, 26.04 mmol) in concentrated sulfuric acid (150 mL) under inert atmosphere was added N-bromosuccinimide (4.6 g, 26.04 mmol) portion wise at RT and heated to 60° C. and stirred for 16 h. The reaction was poured into ice-cold water (100 mL) and extracted with EtOAc (2×100 mL). The combined organic extracts were dried over sodium sulfate, filtered and concentrated in vacuo to obtain the crude. The crude was purified through silica gel column chromatography using 2% EtOAc/hexanes to afford compound 128 (4.9 g, 70%). TLC: 5% EtOAc/hexanes (Rf 0.5); ¹H NMR (DMSO-d₆, 400 MHz): δ 8.11 (d, /=8.8 Hz, 1H), 7.79 (d, /=8.9 Hz, 1H).

Step 2: Synthesis of methyl 3-amino-2,4-dichlorobenzoate (7-3)

To a stirring solution of compound 7-2 (7.5 g, 27.77 mmol) in MeOH: CH₃CN (4: 1, 100 mL) under inert atmosphere in a steel bomb were added triethylamine (12 mL, 83.33 mmol), dppf (1.53 g, 2.76 mmol), Pd(OAc)₂ (500 mg, 2.27 mmol) at RT; heated to 100° C., under CO gas atmosphere (150 psi) and stirred for 16 h. The reaction was monitored by TLC; after completion of the reaction, the reaction mixture was filtered through celite and the filtrate was concentrated in vacuo to obtain the crude. The crude was purified through silica gel column flash chromatography using 15% EtOAc/hexanes to afford compound 7-3 (5 g, 82%). TLC: 30% EtOAc/hexanes (RT. 0.5); ¹H NMR (DMSO-d₆, 400 MHz): δ 7.34 (d, /=8.1 Hz, 1H), 6.94 (d, /=8.1 Hz, 1H), 5.79 (s, 2H), 3.83 (s, 3H).

Step 3: Synthesis of methyl 2,4-dichloro-3-nitrobenzoate (7-4)

To a stirring solution of compound 7-3 (5 g, 22.72 mmol) in glacial acetic acid (25 mL) was added 30% ¾ (¾ (25 mL) at 0° C.; warmed to RT and stirred for 16 h. The reaction was monitored by TLC: after completion of the reaction, the reaction mixture was diluted with water (100 mL) and EtOAc (200 mL). The organic extract was dried over sodium sulfate, filtered and concentrated in vacuo to afford compound 7-4 (4.1 g, 73%) as brown solid. TLC: 30% EtOAc/hexanes (R/. 0.3); ¹H NMR (DMSO-d₆, 400 MHz): δ 8.11 (d, /=8.5 Hz, 1H), 7.96 (d, /=8.7 Hz, 1H), 3.91 (s, 3H).

Step 4: Synthesis of methyl 2-chloro-4-((2-(methoxycarbonyl) phenyl) thio)-3-nitrobenzoate (7-5)

To a stirring solution of compound 7-4 (4.1 g, 16.40 mmol) in DMF (100 mL) under argon atmosphere were added methyl 2-mercaptobenzoate (2.75 g, 16.40 mmol), cesium carbonate (16 g, 49.23 mmol) at 0° C.; warmed to RT and stirred for 16 h. Tire reaction was monitored by TLC: after completion of the reaction, the reaction mixture was diluted with water (500 mL) and extracted with EtOAc (200 mL). The organic extract was dried over sodium sulfate, filtered and concentrated in vacuo to compound 7-5 (1 g, 16%) as while solid. TLC: 30% EtOAc/hexanes (R/. 0.3). ¹H NMR (DMSO-d₆, 400 MHz): δ 8.08 (d, /=8.3 Hz, 1H), 7.98 (dd, /=7.8, 1.5 Hz, 1H), 7.73 (d, /=8.3 Hz, 1H), 7.58-7.52 (m, 1H), 7.43 (td, /=7.6, 1.1 Hz, 1H), 7.02 (dd, /=8.0, 0.6 Hz, 1H), 3.92 (s, 3H), 3.86 (s, 3H).

Step 5: Synthesis of methyl 3-amino-2-chloro-4-((2-(methoxycarbonyl) phenyl) thio) benzoate (7-6)

To a stirring solution of compound 7-5 (1 g, 2.62 mmol) in acetic acid (10 mL) was added iron powder (734 mg, 13.12 mmol) at RT; heated to 60° C. and stirred for 4 h. The reaction was monitored by TLC; after completion of the reaction, the volatiles were removed in vacuo and the residue was diluted with EtOAc (200 mL). The organic layer was washed with saturated sodium bicarbonate solution (100 mL) and dried over sodium sulfate, filtered and concentrated in vacuo to afford compound 7-6 (700 mg, 76%) as brown syrup. TLC: 40% EtOAc/hexanes (R/. 0.7). ¹H NMR (DMSO-d₆, 500 MHz): δ 7.97 (br d, /=7.5 Hz, 1H), 7.47-7.42 (tn. 2H), 7.26 (br t, /=7.5 Hz, 1H), 6.97 (d, /=8.1 Hz, 1H), 6.65 (br d, /=8.1 Hz, 1H), 5.76-5.73 (m, 2H), 3.89 (s, 3H), 3.87 (s, 3H); LC-MS: 90.61%; 351.8 (M⁺+1): (column; Ascentis Express C18, (50×3.0 mm, 2.7 μm); RT 2.82 min. 0.025% Aq. TFA+5% ACN: ACN+5% 0.025% Aq. TFA, 1.2 mL/min).

Step 6: Synthesis of 3-amino-4-((2-carboxyphenyl) thio)-2-chlorobenzoic Acid (7-7)

To a stirring solution of compound 7-6 (700 mg, 1.99 mmol) in THF: ¾ 0 (1: 1, 20 mL) was added lithium hydroxide monohydrate (837 mg, 19.94 mmol) portion wise for 10 min at RT and stirred for 16 h. The reaction was monitored by TLC: after completion of the reaction, the volatiles were removed in vacuo. The pH of the residue was adjusted to ˜2 with I N HCl and extracted with EtOAc (2×50 mL) The organic layer was dried over sodium sulfate, filtered and concentrated in vacuo to compound 7-7 (500 mg, 78%) as a white solid. TLC: 40% EtOAc/hexanes (Rf. 0.2). H NMR (DMSO-d₆, 400 MHz): δ 13.29 (br s, 2H), 7.97 (dd, /=7.8, 14 Hz, 1H), 7.44-7.38 (m, 2H), 7.26-7.21 (m, 1H), 6.95 (d, /=7.9 Hz, 1H), 6.63 (d, /=7.6 Hz, 1H), 5.62 (br s, 2H): LC-MS: 94.65%: 323.9 (M⁺+1); (column; Ascends Express CIS. (50×3.0 mm, 2.7 μm); RT 1.98 min. 0.025% Aq. TFA+5% ACN: ACN+5% 0.025% Aq. TEA. 1.2 mL/min).

Step 7: Synthesis of 9-chloro-ll-oxo-lO, 11-dihydrodibenzo [b,f] [1, 4] thiazepine-8-carboxylic Acid (7-8)

To a stirring solution of compound 7-7 (500 mg, 1.42 mmol) in THF (10 mL) under inert atmosphere was added CDI (2.30 g, 14.20 mmol) at RT and stirred for 16 h. The reaction was monitored by TLC; after completion of the reaction, the volatiles were removed in vacuo. The pH of the residue w as adjusted to ˜2 using 1N HCl. The precipitated solid was filtered, washed with hexane (20 mL) and dried in vacuo to afford compound 7-8 (300 mg, 69%) as an off-white solid. TLC: 10% MeOH/CH₂Cl₂ (Rf. 0.4); ¹H NMR (DMSO-d₆, 400 MHz): δ 13.62 (br s, 1H), 10.41 (s, 1H), 7.72-7.63 (m, 2H), 7.58-7.54 (m, 1H), 7.53-7.46 (m. 3H); LC-MS: 93.51%; 305.9 (M⁺+1); (column; Ascends Express C18. (50×3.0 mm. 2.7 μm); RT 2.02 min. 0.025% Aq. TFA+5% ACN: ACN+5%0.025% Aq. TFA. 1.2 mL/min).

Step 8: Synthesis of 9-chloro-ll-oxo-10, 11-dihydrodibenzo [b,f] [1, 4] thiazepine-8-carboxylic Acid 5,5-dioxide (7-9)

To a stirring solution of compound 7-8 (290 mg, 0.95 mmol) in l. 2 dichloro ethane: C¾CN: ¾ 0 (1:1:2, 20 mL) were added sodium metaperiodate (622 mg, 2.85 mmol), ruthenium chloride (10.70 mg, 0.047 mmol) at RT and stirred for 16 h. The reaction was monitored by TLC; after completion, the volatiles were removed in vacuo and the residue was extracted with EtOAc (2×75 mL). The combined organic extracts were dried over sodium sulfate, filtered and concentrated in vacuo to obtain the erode. The crude was purified through silica gel column chromatography using 10% MeOH/CH2CI2 to afford compound 7-9 (230 mg, 72%) as brown solid. TLC. 40% MeOH/CH₂Cl₂(Rf. 0.3); ¹H NMR (DMSO⁻¹ ¾, 400 MHz). δ 14.08 (br s, 1H), 11.13 (s, 1H), 8.01 (d, /=8.2 Hz, 1H), 7.98-7.89 (m, 3H), 7.85 (dd, /=7.5, 1.4 Hz, 1H), 7.71 (d, /=8.2 Hz, 1H): LC-MS: 99.61%: 335.9 (M−1)⁺; (column; Kinctex EVO C-18 (50×3.0 mm, 2.6 urn); RT 1.15 min. 2.5 mM Aq. NH₄OOCH+5% ACN: ACN+5% 2.5 mM Aq.NH₄OOCH, 0.8 mL/min).

Example 8: Synthesis 9-methyl-ll-oxo-10, 11-dihydrodibenzo [b,f] [1, 4] thiazepine-8-carboxylic Acid 5, 5-dioxide (8-9): a Common Intermediate

Step 1: Synthesis of 1-bromo-4-fluoro-2-methyl-3-nitrobenzene (8-2)

To 1-fluoro-3-methyl-2-nitrobenzene 8-1 (5 g, 32.25 mmol) at 0° C. under argon atmosphere was added concentrated sulfuric acid: trifluoroacetic acid (1: 2.45 mL). To this was added N-bromosuccinimide (8.61 g, 48.37 mmol) portion wise for 15 min: warmed to RT and stirred for 5 h. The reaction was monitored by TLC; after completion of the reaction, lire reaction mixture was poured into ice-cold water (200 mL), the precipitated solid was filtered, washed with water (100 mL) and dried in vacuo to afford the crude. The crude was purified through silica gel flash column chromatography using 1-2% EtOAc/hexanes to afford compound 8-2 (5.1 g. 68%). TLC: 5% EtOAc/hexanes (R/. 0.8): TLC: 30% EtOAc/hexanes (R/. 0.3). ¹H NMR (DMSO-d₆, 400 MHz): δ 7.% (dd, /=8.9, 5.2 Hz, 1H), 7.47 (t, /=9.2 Hz, 1H), 22.35 (m, 3H);

Step 2: Synthesis of methyl 2-((4-bromo-3-methyl-2-nitrophenyl) thio) benzoate (8-3)

To a stirring solution of compound 8-2 (5.1 g, 21.79 mmol) in DMF (80 mL) under argon atmosphere were added cesium carbonate (10.62 g, 32.67 mmol), methyl 2-mercaptobenzoate 1 (4.03 g, 23.97 mmol) at RT and stirred for 2 h. The reaction was monitored by TLC: after completion of the reaction, the reaction mixture was diluted with ice-cold water (100 mL), the precipitated solid was filtered, washed with hexane (100 mL) and diethyl ether (100 mL) and dried in vacuo to afford compound 8-3 (7.0 g, 84%) as an off-white solid. TLC: 10% EtOAc/hexanes (Rf. 0.3); ¹H NMR (DMSO-d₆, 400 MHz): δ 8.09-7.85 (m, 2H), 7.55-7.46 (m, 2H), 7.34 (td, /=7.6, 1.1 Hz, 1H), 6.81 (dd, /=8.2, 0.8 Hz, 1H), 3.87 (s, 3H), 2.35 (s, 3H); LC-MS: 98.98%; 383.2 (M+2)˜; (Column; X-select CSH C-18 (50×3.0 mm, 2.5 um); RT 4.99 min. 2.5 mM Aq. NH₄OAc:ACN, 0.8 mL/min).

Step 3: Synthesis of methyl 2-((2-amino-4-bromo-3-methylphenyl) thio) benzoate (8-4)

To a stirring solution of compound 8-3 (7 g, 18.32 mmol) in acetic acid (100 mL) was added iron powder (10.2 g, 182.7 mmol) at RT; heated to 80° C. and stirred for 16 h. The reaction was monitored by TLC and LC-MS; after completion of the reaction, the reaction mixture was filtered through celite, the filtrate was concentrated in vacuo. The residue was diluted with EtOAc (200 mL), washed with water (2×100 mL). The organic extract was dried over sodium sulfate, filtered and concentrated in vacuo to compound 8-4 (5.8 g, 90%) as an off-white solid. TLC: 10% EtOAc/hexanes (Rf. 0.2); LC-MS: 98.31%; 353.9 (M⁻+2); (column; Ascentis Express C18. (50×3.0 mm, 2.7 μm): RT 3.06 min. 0.025% Aq. TFA+5% ACN: ACN+5% 0.025% Aq TFA, 1.2 mL/min).

Step 4: Synthesis of 2-((2-amino-4-bromo-3-methylphenyl) thio) benzoic Acid (8-5)

To a stirring solution of compound 8-4 (4.8 g, 13.63 mmol) in THF: ¾ 0 (3: 1, 120 mL) was added lithium hydroxide monohydrate (1.72 g, 40.95 mmol) at RT and stirred for 16 h. The reaction was monitored by TLC; after completion of the reaction, the volatiles were removed in vacuo. The residue was diluted with water (20 mL) and acidified with 2 N HC) to pH −4-5. The obtained solid was filtered, washed with (50 mL) and dried in vacuo to obtain compound 8-5 (4 g, 87%) as an off-white solid. TLC. 20% EtOAc/hexanes (Rf. 0.2); LC-MS: 98.82%; 339.9 (M*+2); (column; Ascentis Express C18, (50×3.0 mm, 2.7 μm). RT 2.67 min. 0.025% Aq. TFA+5% ACN: ACN+5% 0.025% Aq. TFA. 1.2 mL/min).

Step 5: Synthesis of 8-bromo-9-methyldibenzo [b,f] [1, 4] thiazepin-ll(10H)-one (8-6)

To a stirring solution of compound 8-5 (4.7 g, 13.90 mmol) in THF (100 mL) under inert atmosphere was added CDI (13.50 g, 83.32 mmol) at RT and stirred for 24 h. The reaction was monitored by TLC; after completion of the reaction, the volatiles were removed in vacuo. The pH of the residue was adjusted to ˜2 using 2 N HCl. The precipitated solid was filtered, washed with water (50 mL) and dried in vacuo to afford compound 8-6 (3 g, 68%) as white solid. TLC: 30% EtOAc/hexanes (Rf 0.4) ¹H NMR (DMSO-d₆, 400 MHz): δ 10.36 (s, 1H), 7.68-7.63 (m, 1H), 7.54-7.49 (m, 1H), 7.49-7.36 (m, 4H), 2.41 (s, 3H);

Step 6: Synthesis of methyl 9-methyl-ll-oxo-10, 11-dihydrodibenzo [b,f] [1, 4]thiazepine-8-carboxylate (8-7)

To a stirring solution of compound 8-6 (1.5 g, 4.68 mmol) in MeOH (30 mL) in a steel bomb under inert atmosphere were added dppf (259 mg, 0.46 mmol), sodium acetate (1.15 g, 14.02 mmol). Pd(OAc)₂ (105 mg, 0.46 mmol) at RT and heated to 100° C. under CO gas atmosphere (150 psi) and stirred for 24 h. The reaction was monitored by TLC, alter completion of the reaction, the reaction mixture was filtered through celite. The filtrate was concentrated in vacuo to obtain the crude. The crude was purified through silica gel column flash chromatography using 10-20% EtOAc/hexanes to afford compound 8-7 (11 g, 79%). TLC: 20% EtOAc/hexanes (RT 0.2); LC-MS: 98.18%; 299.9 (M+1)⁺; (column; Ascends Express C18, (50×3.0 mm. 2.7 μm), RT 2.38 min. 0.025% Aq. TFA+5% ACN: ACN+5% 0.025% Aq. TFA, 1.2 mL/min).

Step 7: Synthesis of methyl 9-methyl-ll-oxo-10, 11-dihydrodibenzo [b,f] [1, 4]thiazepine-8-carboxylate 5,5-dioxide (8-8)

To a compound 8-7 (1.1 g, 3.67 mmol) in 1, 2 dichloro ethane:CH₃CN:H2O (1:1:2, 40 mL) were added sodium metaperiodate (2.35 g, 11.03 mmol), ruthenium chloride (38 mg, 0.18 mmol) at RT and stirred for 16 h. The reaction was monitored by TLC; alter completion, the reaction mixture was diluted with ice-cold water (50 mL) and extracted with EtOAc (2×75 mL). The combined organic extracts were dried over sodium sulfate, filtered and concentrated in vacuo to afford compound 8-8 (1 g, 83%) as an white solid. TLC: 40% EtOAc/hexanes (Rf. 0.2): ¹H NMR (DMSO-d₆, 400 MHz): δ 10.87 (s, 1H), 7.95-7.84 (m, 4H), 7.83-7.78 (m, 1H), 7.68 (d, /=8.3 Hz, 1H), 3.85 (s, 3H), 2.45 (s, 3H): LC-MS: 98.10%: 332.0 (M+1)⁺; (column; Ascends Express C18, (50×3.0 mm, 2.7 μm); RT 2.16 min. 0.025% Aq. TFA+5% ACN: ACN+5% 0.025% Aq TFA, 1.2 mL/min).

Step 8: Synthesis of 9-methyl-ll-oxo-10, 11-dihydrodibenzo [b,f] [1, 4] thiazepine-8-carboxylic Acid 5,5-dioxide (8-9)

To a stirring solution of compound 8-8 (1.07 g, 3.23 mmol) in THF: ¾ 0 (3:1, 18 mL) was added lithium hydroxide monohydrate (407 mg, 9.69 mmol) at 0° C.; warmed to RT and stirred for 16 h. The reaction was monitored by TLC; alter completion of the reaction, the volatiles were removed in vacuo. The pH of the residue was acidified with 2 N HCl to ˜2. The precipitated solid was filtered, washed with water (50 mL), hexane (20 mL) and dried in vacuo to afford 8-9 (950 mg, 93%) as white solid. TLC: 5% MeOH/CH₂Cl₂ (Rf. 0.1); ¹H NMR (DMSO-4, 400 MHz): δ 3.63 (br s, 1H), 10.85 (s, 1H), 7.96-7.84 (m, 4H), 7.83-7.78 (m, 1H), 7.67 (d, /=8.1 Hz, 1H), 2.48 (s, 3H); LC-MS: 98.67%; 317.9 (M+1)⁺; (column; Ascentis Express C18, (50×3.0 mm, 2.7 μm); RT 1.81 min. 0.025% Aq. TFA+5% ACN: ACN+5% 0.025% Aq. TFA, 1.2 mL/min).

Example 9: 9-methyl-ll-oxo-N-((2-(trifluoromethyl)thiazol-5-yl)methyl)-10,ll-dihydrodibenzo[b,f][1,4]thiazepine-8-carboxamide 5,5-dioxide (9-2)

Route 1 Step 1

Compound 8-7a was obtained from compound 8-7 by hydrolysis of the methyl ester. Following amide formation procedure described in Example 4 and substituting acid 3-7 with 8-7a, compound 9-1 was obtained. ¹H-NMR (DMSO-d₆, 500 MHz); δ 10.24 (s, 1H), 9.15 (t, /=5.8 Hz, 1H), 8.04 (s, 1H), 7.67-7.63 (m, 1H), 7.54-7.49 (m, 2H), 7.48-7.41 (m, 2H), 7.11 (d, /=7.8 Hz, 1H), 4.69 (d, /=5.8 Hz, 2H), 2.28 (s, 3H); LCMS: m/z 448.1 [M−H].

Route 1 Step 2

Subjecting a sample of 9-1 to oxidative conditions described in Example 3 (Method A or Method B) led to compound 9-2. ¹H-NMR (DMSO-d₆, 400 MHz): δ 10.86 (br s, 1H), 9.30 (t, /=5.8 Hz, 1H), 8.06 (s, 1H), 7.95-7.84 (m, 4H), 7.80 (td, /=7.5, 1.4 Hz, 1H), 7.38 (d, /=8.2 Hz, 1H), 4.72 (d, /=5.6 Hz, 2H), 2.31 (s, 3H); LCMS: m/z: 481.1 [M+H]⁺.

Compound 9-2 was obtained alternatively produced by reaction a sample of acid 8-9 with amine 1-5 using the amide coupling conditions described in Example 4.

Example 10: N-((2-ethylthiazol-5-yl)methyl)-ll-oxo-10,ll-dihydrodibenzo[b,f][1,4]thiazepine-8-carboxamide 5,5-dioxide (10)

The title compound 10 was obtained alternatively produced by reaction a sample of acid 3-7 with amine 2-7 using the amide coupling conditions described in Example 4. ¹H-NMR (DMSO-d₆, 500 MHz): δ 11.52 (s, 1H), 9.40 (t, /=5.8 Hz, 1H), 8.04 (d, /=8.4 Hz, 1H), 7.97 (t, /=8.4 Hz, 2H), 7.90 (t. /=7.1 Hz, 1H), 7.87-7.81 (m, 2H), 7.78 (d, /=8.1 Hz, 1H), 7.53 (s, 1H), 4.58 (d, /=5.8 Hz, 2H), 2.89 (q, /=7.5 Hz, 2H), 1.23 (t, /=7.5 Hz, 3H); LCMS: m/z: 427.9 [M=H]⁺.

Example 11: ll-oxo-N-((2-(trifluoromethyl)thiazol-5-yl)methyl)-10,ll-dihydrodibenzo[b,f][1,4]thiazepine-8-carboxamide 5-oxide (11-3)

Step 1: Synthesis of ll-oxo-10, 11-dihydrodibenzo [b,f] [1,4] thiazepine-8-carboxylic Acid 5-oxide (89)

To a stirring solution of 11-1 (obtained similarly by following procedures described in the Examples 3, 5, 7, 8) (2.5 g, 9.21 mmol) in CH2CI2 (50 mL) under inert atmosphere was added m-chloro perbenzoic acid (1.59 g, 9.21 mmol) at RT and stirred for 48 h. The reaction was monitored by TLC; after completion of the reaction, the reaction mixture was the volatiles were removed in vacuo to obtain the crude. The crude was triturated with 10% MeOH/CH2CI2 (2×5 mL), isopropanol (10 mL) to afford compound 11-2 (2.3 g, 87%) as white solid. TLC: 10*/. MeOH/CH₂Cl₂+0.05 mL CH₃COOH (R/. 0.4); ¹H NMR (DMSO-4, 500 MHz): δ 1.3.36 (br s, 1H), 11.08 (s, 1H), 7.96 (d, /=7.8 Hz, 1H), 7.92-7.87 (m, 1H), 7.85-7.66 (m, 3H), 7.63 (t, /=7.8 Hz, 1H), 7.53 (t. /=7.8 Hz, 1H);

Step 2: ll-oxo-N-((2-(trifluoromethyl)thiazol-5-yl)methyl)-10,ll-dihydrodibenzo[b,f][1,4]thiazepine-8-carboxamide

5-oxide was prepared by coupling 11-1 and amine 1-5 following procedures described in Example 4. ¹H-NMR (DMSO-d₆, 400 MHz): δ 11.06 (s, 1H), 9.46 (t, /=5.8 Hz, 1H), 8.05 (s, 1H), 7.88 (dd. /=8.2, 1.5 Hz, 1H), 7.84-7.77 (m, 2H), 7.76-7.68 (m, 3H), 7.63 (td, /=7.5, 13 Hz, 1H), 4.72 (d, /=5.6 Hz, 2H); LCMS: m/z: 451.9 [M+H]⁺.

INCORPORATION BY REFERENCE

All publications and patents mentioned herein, including those items listed below, are hereby incorporated by reference in their entirety for all purposes as if each individual publication or patent was specifically and individually incorporated by reference. In case of conflict, the present application, including any definitions herein, w ill control.

EQUIVALENTS

While specific embodiments of the subject invention have been discussed, the above specification is illustrative and not restrictive. Many variations of the invention will become apparent to those skilled in the an upon review of this specification. The full scope of the invention should be determined by reference to the claims, along with their full scope of equivalents, and the specification, along with such variations.

Unless otherwise indicated, all numbers expressing quantities of ingredients, to reaction conditions, and so forth used in the specification and claims are to be understood as being modified in all instances by the term “about.” Accordingly, unless indicated to the contrary, the numerical parameters set forth in this specification and attached claims are approximations that may vary depending upon the desired properties sought to be obtained by the present invention. 

What is claimed is: 1.-17. (canceled)
 18. A process for preparing a compound represented by Formula VIII:

wherein w is 0, 1 or 2; A is a 5-6 membered monocyclic heteroaryl having 1, 2 or 3 heteroatoms each independently selected from the group consisting of S, N, and O; wherein said heteroaryl may optionally be substituted with one or more substituents selected from R^(f); R^(f) is independently selected, for each occurrence, from the group consisting of hydrogen, halogen, hydroxyl, cyano, R^(a)R^(b)N—, R^(a)R^(b)N-carbonyl-, R^(a)R^(b)N—SO₂—, C₁₋₆alkyl, C₃₋₆cycloalkyl, C₂₋₆alkenyl, C₂₋₆alkynyl, C₁₋₆alkoxy, C₁₋₆alkyl-S(O)_(w)—, (wherein w is 0, 1 or 2), C₁₋₆alkylcarbonyl-N(R^(a))— and C₁₋₆alkoxycarbonyl-N(R^(a))—; wherein C₁₋₆alkyl, C₃₋₆cycloalkyl, C₂₋₆alkenyl, C₂₋₆alkynyl, C₁₋₆alkoxy, C₁₋₆alkyl-S(O)_(w)—, C₁₋₆alkylcarbonyl-N(R^(a))—, C₁₋₆alkoxycarbonyl-N(R^(a))— may be optimally substituted by one or more substituents selected from the group consisting of halogen, hydroxyl, and C₁₋₆alkoxy; R^(a) and R^(b) are independently selected, for each occurrence, from the group consisting of hydrogen and C₁₋₃alkyl; wherein C₁₋₃alkyl may optionally be substituted by me or more substituents selected from fluorine, hydroxyl, C₁₋₃alkoxy; or R^(a) and R^(b), together with the nitrogen to which they are attached, may form a 4-6 membered heterocyclic ring; comprising: coupling a compound of Formula IX:

with a compound of Formula X:

to provide a compound of Formula XI:

optionally oxidizing the compound of Formula XI to provide a compound of Formula XII:

wherein w is 1 or 2; reducing the compound of Formula XII to provide a compound of Formula XIII:

wherein w is 1 or 2; hydrolyzing the compound of Formula XIII to provide a compound of Formula XIV:

cyclizing the compound of Formula XIV to provide a tricyclic compound of Formula VII:

and amidating the compound of Formula VII to provide the compound of Formula VIII.
 19. The process of claim 18, wherein coupling the compound of Formula IX with the compound of Formula X is conducted in the presence of a base.
 20. The process of claim 18, wherein the base is cesium carbonate.
 21. The process of claim 18, wherein oxidizing the compound of Formula XI comprises contacting the compound of Formula XI with aqueous hydrogen peroxide.
 22. The process of claim 18, wherein oxidizing the compound of Formula XI comprises contacting the compound of Formula XI with sodium metaperiodate and catalytic ruthenium (III) chloride.
 23. The process of claim 18, wherein reducing the compound of Formula XII comprises contacting the compound of Formula XII with Pd/C under hydrogen gas.
 24. The process of claim 18, wherein hydrolyzing the compound of Formula XIII comprises contacting the compound of Formula XIII with an aqueous base.
 25. The process of claim 24, wherein the aqueous base is aqueous lithium hydroxide.
 26. The process of claim 18, wherein cyclizing the compound of Formula XIV comprises contacting the compound of Formula XIV with carbodiimidazole.
 27. The process of claim 18, wherein amidating the compound of Formula VII comprises contacting the compound of Formula VII with a primary amine represented by A-CH₂—NH₂ in the presence of a coupling agent.
 28. The process of claim 27, wherein the coupling agent is hydroxy benzotriazole/ethyl-(N′,N′-dimethylamino)propylcarbodiimide hydrochloride.
 29. The process of claim 27, wherein the primary amine is represented by:

wherein R^(f) is C₁₋₆alkyl optionally substituted by one or more fluorine atoms.
 30. The process of claim 27, wherein the primary amine is:


31. The process of claim 18, wherein the compound of Formula VII is:


32. The process of claim 18, wherein the compound of Formula VIII is:


33. A compound or pharmaceutically acceptable salt thereof, represented by:

prepared by the process of claim
 18. 